LY6 anticorps (Alexa Fluor 647)

N° du produit ABIN7840926

Cet anticorps Rat Monoclonal détecte spécifiquement LY6 dans FACS et IF. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour LY6 anticorps (Alexa Fluor 647) (ABIN7840926)

Antigène: LY6 (Lymphocyte Antigen 6 Complex (LY6))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LY6 est conjugé à/à la Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: RB6-8C5

Détail du produit anti-LY6 anticorps (Alexa Fluor 647)

Fonction: Rat monoclonal antibody AF647 labled to Ly-6G/C

Specificité: Recognizes mouse Ly-6G/C

Purification: The antibody was purified by affinity chromatography.

Immunogène: Raised against granulocytes of mouse origin

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur LY6

Antigène: LY6 (Lymphocyte Antigen 6 Complex (LY6))

Autre désignation: Ly6

Sujet: Lymphocyte antigen 6C1, Ly-6C1, Lymphocyte antigen 6G, Ly-6G

ID gène: 17067