Recombinant MGMT anticorps (AA 1-100)

N° du produit ABIN7864246

Cet anticorps Lapin Monoclonal détecte spécifiquement MGMT dans WB et IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant MGMT anticorps (AA 1-100) (ABIN7864246)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MGMT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: MGMT-8364R

Détail du produit anti-MGMT anticorps

Épitope: AA 1-100

Fonction: Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

Specificité: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal crossT 79oΩ 79oΩlinks at the O6T 79oΩ 79oΩ alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6T 79oΩ 79oΩposition of guanine in DNA (prior to crossT 79oΩ 79oΩlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNAT 79oΩ 79oΩdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa1-100) of human MGMT (exact sequence is proprietary)

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MGMT

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

Sujet:

6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase

Jurkat, MCF-7, LNCaP, Hep G2 and human liver. Human tonsil or stomach (IHC).

Poids moléculaire: 21.646 kDa

ID gène: 4255, 501522

UniProt: P16455

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus