Recombinant Cyclin B1 anticorps

N° du produit ABIN7861160

Cet anticorps Lapin Monoclonal détecte spécifiquement Cyclin B1 dans IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Cyclin B1 anticorps (ABIN7861160)

Antigène: Cyclin B1 (CCNB1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: CCNB1-9242R

Détail du produit anti-Cyclin B1 anticorps

Fonction: Recombinant Cyclin B1 (G2- & M-phase Cyclin) Antibody

Specificité: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephos phorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physio logical substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: His-tagged recombinant hamster CCNB1 protein

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: Cyclin B1

Sujet:

CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Cell line in logarithmic growth phase. Human tonsil|Prostate or Ovarian Carcinoma.

Poids moléculaire: 55-62kDa

ID gène: 891, 23960

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase