Recombinant MLH1 anticorps

N° du produit ABIN7861776

L’anticorps anti-MLH1 Monoclonal Lapin est utilisé pour la détection de MLH1 dans des échantillons de Humain. Il a été validé pour WB et IHC.

Aperçu rapide pour Recombinant MLH1 anticorps (ABIN7861776)

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MLH1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: MLH1-8855R

Détail du produit anti-MLH1 anticorps

Fonction: Recombinant MLH1 (MutL Homolog 1) / HNPCC Antibody

Specificité: This MAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). 79oΩHeterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human MLH1 protein

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MLH1

Sujet:

COCA2, DNA mismatch repair protein, FCC2, hMLH1, MutL homolog 1 colon cancer nonpolyposis type 2 (HNPCC2)

Human tonsil or colon carcinoma. HeLa, A431

Poids moléculaire: 85kDa

ID gène: 4292, 195364

UniProt: P40692

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response