MGMT anticorps

N° du produit ABIN7863130

L’anticorps anti-MGMT Monoclonal Souris est utilisé pour la détection de MGMT dans des échantillons de Humain. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour MGMT anticorps (ABIN7863130)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MGMT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: MGMT-4791

Détail du produit anti-MGMT anticorps

Fonction: MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

Specificité: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross links at the O6 alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 position of guanine in DNA (prior to cross link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Purified His-tagged MGMT protein

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MGMT

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

Sujet:

6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase

Jurkat or MCF-7 cells. Human tonsil or stomach (IHC).

Poids moléculaire: 21.646 kDa

ID gène: 4255, 501522

UniProt: P16455

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus