Recombinant MGMT anticorps

N° du produit ABIN7861875

L’anticorps anti-MGMT Monoclonal Lapin est utilisé pour la détection de MGMT dans des échantillons de Humain. Il a été validé pour WB et IHC.

Aperçu rapide pour Recombinant MGMT anticorps (ABIN7861875)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MGMT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: MGMT-8319R

Détail du produit anti-MGMT anticorps

Fonction: Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

Specificité: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross 79oΩlinks at the O6 79oΩ alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 79oΩposition of guanine in DNA (prior to cross 79oΩlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA 79oΩdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Purified His-tagged MGMT protein

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Détails sur MGMT

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

Sujet:

6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase

MCF-7, LNCaP, Hep G2 and human liver, Jurkat. Human tonsil or stomach (IHC).

Poids moléculaire: 21.646 kDa

ID gène: 4255, 501522

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus