ube3a anticorps

N° du produit ABIN7863487

Cet anticorps Souris Monoclonal détecte spécifiquement ube3a dans IF et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour ube3a anticorps (ABIN7863487)

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ube3a est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-UBE3A-1A2

Détail du produit anti-ube3a anticorps

Fonction: UBE3A / E6-AP Antibody

Specificité: E6-associating protein (E6-AP), also designated ubiquitin protein ligase E3A(UBE3A), is a component of the ubiquitin-mediated proteolytic pathway thatselectively targets proteins for degradation by the 26S Proteasome. Ubiquitin(Ub) is directly conjugated to protein substrates by the transfer of Ub from anE2 ubiquitin conjugating enzyme to the target protein. This conjugation is facil-itated by the enzymatic activity of E3 ubiquitin ligase family members such asE6-AP. Several substrates of E6-AP have been identified and include the tumorsuppressor protein p53 and the mammalian homolog of Rad23, HHR23A.Previous studies have indicated that E6-AP associates with the humanpapillomavirus E6 oncogene, which forms a complex with p53 and therebypotentiates E6-AP mediated ubiquitination of p53. Genetic mutations thatimpair E6-AP activity result in the accumulation of p53 in the cytoplasm, andin many instances, these mutations are associated with the development ofthe rare neurodevelopmental disorder Angelman syndrome (AS), which ischaracterized by severe motor dysfunction and mental retardation.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human UBE3A protein

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur ube3a

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Autre désignation: UBE3A

Sujet:

ANCR, Angelman syndrome (AS), CTCL tumor antigen se37-2, E6AP ubiquitin protein ligase, EPVE6AP, FLJ26981, HECT-type ubiquitin transferase E3A, Human papilloma virus E6 associated protein Angelman syndrome, HPVE6A, NY REN 54 antigen, Oncogenic protein associated protein E6AP, Renal carcinoma antigen NY REN 54,

HeLa cells.

Poids moléculaire: 100kDa

ID gène: 7337, 598862

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway