DNA-RNA Hybrid anticorps

N° du produit ABIN7862329

Cet anticorps anti-DNA-RNA Hybrid Monoclonal Souris (Clone S9-6) (ABIN7862329) détecte spécifiquement DNA-RNA Hybrid dans ChIP, ICC, IF, FACS et IP. L’anticorps est réactif avec des échantillons de Différentes espèces et Humain.

Aperçu rapide pour DNA-RNA Hybrid anticorps (ABIN7862329)

Antigène: DNA-RNA Hybrid

Reactivité: Différentes espèces, Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: S9-6

Détail du produit

Fonction: DNA-RNA Hybrid Antibody

Specificité: We have not tested this antibody in-house in Immunofluorescence, CHIP, Immunocytochemistry, Immunoprecipitation or Flow Cytometry. All application recommendations come from publications using this clone. DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate.

Immunogène: DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Détail du antigène

Antigène: DNA-RNA Hybrid

Sujet:

DNA-RNA Duplex, DNA/RNA Duplex, DNA:RNA Duplex, DNA-RNA Hybrid, DNA/RNA Hybrid, DNA:RNA Hybrid, RNA-DNA Duplex, RNA/DNA Duplex, RNA:DNA Duplex, RNA-DNA Hybrid, RNA/DNA Hybrid, RNA:DNA Hybrid

All. Target DNA-RNA heteroduplex (R loop) structure is not sequence-specific or species-specific.