DNA-RNA Hybrid anticorps

N° du produit ABIN7880638

Cet anticorps anti-DNA-RNA Hybrid Monoclonal Souris (Clone S9-6) (ABIN7880638) détecte spécifiquement DNA-RNA Hybrid dans ChIP, ICC, IF et FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour DNA-RNA Hybrid anticorps (ABIN7880638)

Antigène: DNA-RNA Hybrid

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: S9-6

Détail du produit

Fonction: DNA-RNA Hybrid Antibody

Purification: Protein G affinity

Immunogène: A DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase was used as the immunogen for the DNA-RNA Hybrid antibody.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal dilution of the DNA-RNA Hybrid antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the DNA-RNA Hybrid antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: DNA-RNA Hybrid

Sujet: We have not tested this antibody in-house in Immunofluorescence, CHIP, Immunocytochemistry, Immunoprecipitation or Flow Cytometry. All application recommendations come from publications using this clone.

DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. A locus forming an DNA:RNA creates a double-stranded A/B intermediate conformation, with a second target for single-stranded nucleic acid binding proteins on the complementary, displaced DNA strand. They are shown to be resistant to the activity of DNA methyltransferases. The formation of DNA:RNA hybrids has been associated with a number of neurological diseases. Mutations in the DNA:RNA helicase senataxin (SETX) are implicated in the dominant juvenile form of amyotrophic lateral sclerosis type 4 and a recessive form of ataxia oculomotor apraxia type 2. Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).