Ubiquitin anticorps (acLys27)

N° du produit ABIN7862469

L’anticorps anti-Ubiquitin Polyclonal Lapin est utilisé pour la détection de Ubiquitin dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour Ubiquitin anticorps (acLys27) (ABIN7862469)

Antigène: Ubiquitin (Ub)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ubiquitin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-Ubiquitin anticorps

Épitope: acLys27

Fonction: Rabbit Anti-Ub (Acetyl Lys27) Polyclonal Antibody

Specificité: Acetyl-Ub (K27) Polyclonal AntibodySynthesized peptide derived from the human Ub around the acetylation site of K27.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the human Ub around the acetylation site of K27

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur Ubiquitin

Antigène: Ubiquitin (Ub)

Autre désignation: Ub

Sujet: UBB, Polyubiquitin-B, UBC, Polyubiquitin-C, RPS27A, UBA80, UBCEP1, Ubiquitin-40S ribosomal protein S27a, Ubiquitin carboxyl extension protein 80, UBA52, UBCEP2, Ubiquitin-60S ribosomal protein L40, CEP52, Ubiquitin A-52 residue ribosomal protein fusion prUbiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27.

UniProt: P62987, P62979, P0CG47, P0CG48

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway