NUMA1 anticorps

N° du produit ABIN7865708

Cet anticorps Souris Monoclonal détecte spécifiquement NUMA1 dans WB, IHC et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour NUMA1 anticorps (ABIN7865708)

Antigène: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NUMA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: EBS-C-002

Détail du produit anti-NUMA1 anticorps

Fonction: anti-NuMA mouse monoclonal, EBS-C-002, purified

Purification: affinity chromatography

Immunogène: live Ls 174T cells (colon carcinoma)

Isotype: IgM kappa

Information d'application

Indications d'application:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur NUMA1

Antigène: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Autre désignation: NuMA

Sujet: EBS-C-002 reacts with NuMA or Nuclear Mitotic Apparatus protein, which at the onset of mitosis redistributes from the nucleus to two centrosomal structures at the poles of the mitotic spindle, where it plays a vital role in establishing and maintaining its bipolar structure. After anaphase the protein redistributes from the spindle polar region into the reforming nucleus and concentrates initially at the site where nuclear lamins and perichomatin have been reported to assemble. In contrast to mitotic cells, post-mitotic neurons display NuMA both in the nucleus and in the cytoplasm. Due to release from dead cells, NuMA is also used as oncological marker in serum and urine. In addition, chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia. Positive control: Human toncil.

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase