PLAP anticorps

N° du produit ABIN7865712

L’anticorps anti-PLAP Monoclonal Souris est utilisé pour la détection de PLAP dans des échantillons de Humain. Il a été validé pour WB, IHC, ELISA, FACS et IP.

Aperçu rapide pour PLAP anticorps (ABIN7865712)

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PLAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: F5C2 (C2)

Détail du produit anti-PLAP anticorps

Fonction: anti-PLAP (Placental alkaline phosphatase) mouse monoclonal, F5C2 (C2), purified

Purification: affinity chromatography

Immunogène: PLAP, purified from FS phenotype human placenta

Isotype: IgG1

Information d'application

Indications d'application:

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur PLAP

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Autre désignation: PLAP (Placental alkaline phosphatase)

Sujet: PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98 % homologous. Non tissue specific AP is found in kidney, liver and bone. F5C2 binds equally well to all common allelic variants (S,F, FS and I) of PLAP and to some variants of AP from normal human testis, while antibody H7E8 reacts with all variants of AP in normal human testis. Positive control: Human placenta.