VEGF anticorps

N° du produit ABIN7865754

L’anticorps anti-VEGF Monoclonal Souris est utilisé pour la détection de VEGF dans des échantillons de Humain, Souris, Rat, Chien et Lapin. Il a été validé pour IHC, IF, FACS et ICC.

Aperçu rapide pour VEGF anticorps (ABIN7865754)

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivité: Humain, Souris, Rat, Chien, Lapin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VEGF est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: VEGF-21

Détail du produit anti-VEGF anticorps

Fonction: anti-Vascular Endothelial Growth Factor (VEGF) mouse monoclonal, VEGF-21, purified

Purification: affinity chromatography

Immunogène: human VEGF189 recombinant protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur VEGF

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Sujet: VEGF-21 reacts with Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor (VEGF/ VPF) and is the key mediator of angiogenesis. The MWs are 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. In addition to endothelial cells, VEGF and VEGF receptors are expressed on numerous non-endothelial cells including tumor cells. Positive control: Astrocytomas, breast or ovarian carcinomas.