MYOD1 anticorps (AA 1-120)

N° du produit ABIN7868018

Cet anticorps Lapin Polyclonal détecte spécifiquement MYOD1 dans WB et ELISA. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MYOD1 anticorps (AA 1-120) (ABIN7868018)

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYOD1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-MYOD1 anticorps

Épitope: AA 1-120

Fonction: MYOD1 Antibody / Myoblast determination protein 1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human MYOD1 recombinant protein (Position: M1-R120) was used as the immunogen for the MYOD1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the MYOD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the MYOD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur MYOD1

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: MYOD1

Sujet: MYOD1 antibody detects Myoblast determination protein 1, a master transcription factor that controls skeletal muscle differentiation. The UniProt recommended name is Myoblast determination protein 1 (MYOD1). This basic helix-loop-helix (bHLH) transcription factor binds DNA at E-box motifs to activate muscle-specific gene expression, converting multipotent mesodermal cells into myoblasts.

Functionally, MYOD1 antibody identifies a 320-amino-acid nuclear protein that dimerizes with E proteins to regulate the transcription of myogenic regulatory genes such as MYOG and MYF5. MYOD1 acts as a pioneer factor, remodeling chromatin to establish muscle-specific transcriptional programs. It is indispensable for myogenic lineage commitment and muscle regeneration following injury.

The MYOD1 gene is located on chromosome 11p15.1 and is expressed in skeletal muscle progenitor cells and differentiating myoblasts. MYOD1 operates in coordination with MEF2 family transcription factors to control muscle fiber formation and repair. During development, its expression defines the onset of the myogenic program.

Pathologically, MYOD1 dysregulation is associated with muscle developmental disorders and certain sarcomas. Mutations in MYOD1 have been identified in rhabdomyosarcoma, where aberrant transcriptional activation promotes tumorigenesis. Research using MYOD1 antibody supports studies in muscle development, differentiation, and cancer biology.

MYOD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect myogenic transcription factors. NSJ Bioreagents provides MYOD1 antibody reagents optimized for skeletal muscle biology, stem cell differentiation, and gene regulation research.

Structurally, Myoblast determination protein 1 contains a bHLH domain that mediates DNA binding and dimerization. Its N-terminal activation domain recruits chromatin remodeling complexes to activate transcription. This antibody facilitates detailed examination of MYOD1's regulatory network in muscle cell fate determination.

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development