PPIH anticorps (AA 1-177)

N° du produit ABIN7868261

Cet anticorps Lapin Polyclonal détecte spécifiquement PPIH dans WB, ELISA, IF et ICC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PPIH anticorps (AA 1-177) (ABIN7868261)

Antigène: PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPIH est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-PPIH anticorps

Épitope: AA 1-177

Fonction: PPIH Antibody / Peptidyl-prolyl cis-trans isomerase H

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human PPIH recombinant protein (Position: M1-M177) was used as the immunogen for the PPIH antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PPIH antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PPIH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PPIH

Antigène: PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Autre désignation: PPIH

Sujet: PPIH antibody detects Peptidyl-prolyl cis-trans isomerase H, a cyclophilin family enzyme that assists in protein folding and pre-mRNA splicing. The UniProt recommended name is Peptidyl-prolyl cis-trans isomerase H (PPIH), also known as Cyclophilin H. PPIH catalyzes the cis-trans isomerization of peptide bonds preceding proline residues, a rate-limiting step in protein folding and conformational regulation.

Functionally, PPIH antibody identifies a 177-amino-acid protein localized primarily in the nucleus, where it associates with the spliceosome machinery. PPIH is a component of the U4/U6.U5 tri-snRNP complex, interacting with PRPF4, SNU13, and other splicing factors. Through its isomerase activity, PPIH facilitates conformational rearrangements necessary for spliceosome assembly and catalysis. Beyond its role in RNA splicing, PPIH also contributes to protein folding and trafficking, reflecting its general chaperone-like activity within the cyclophilin family.

The PPIH gene is located on chromosome 1p34.2 and encodes a protein containing a single cyclophilin-type peptidyl-prolyl isomerase (PPIase) domain. PPIH is structurally similar to other cyclophilins such as PPIA and PPIL1 but exhibits specialized interactions within the spliceosome. Its enzymatic activity accelerates folding of substrates containing cis-proline bonds, influencing the conformation of RNA-binding proteins and enzymes involved in gene expression.

Loss or inhibition of PPIH function affects pre-mRNA splicing efficiency and fidelity, leading to transcriptome-wide alterations. Dysregulation of PPIH expression has been observed in cancer and neurodegenerative diseases, suggesting roles in RNA metabolism and stress response. In cell signaling, PPIH interacts with transcription and export factors, linking splicing to mRNA maturation and nuclear export.

PPIH antibody is used to study RNA processing, protein folding, and chaperone function. It is applicable for immunoblotting, immunoprecipitation, and fluorescence microscopy to examine PPIH localization and protein-protein interactions. In structural biology, PPIH serves as a model enzyme for studying cyclophilin catalytic mechanisms. Its sensitivity to cyclosporine A highlights its functional conservation across cyclophilins and its potential involvement in immunomodulatory pathways.

Structurally, PPIH features a conserved beta-barrel fold typical of cyclophilins, with a hydrophobic active site pocket that accommodates the proline-containing peptide bond. It is post-translationally regulated by phosphorylation and complex assembly within spliceosomal subunits. NSJ Bioreagents provides PPIH antibody reagents validated for research in RNA biology, splicing regulation, and protein folding mechanisms.

UniProt: O43447