UBE2F anticorps (AA 1-185)

N° du produit ABIN7868319

L’anticorps anti-UBE2F Polyclonal Lapin est utilisé pour la détection de UBE2F dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, ELISA, IF, ICC et FACS.

Aperçu rapide pour UBE2F anticorps (AA 1-185) (ABIN7868319)

Antigène: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE2F est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-UBE2F anticorps

Épitope: AA 1-185

Fonction: UBE2F Antibody / NEDD8-conjugating enzyme E2 F

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human UBE2F recombinant protein (Position: M1-R185) was used as the immunogen for the UBE2F antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the UBE2F antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the UBE2F antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur UBE2F

Antigène: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Autre désignation: UBE2F

Sujet: UBE2F antibody detects NEDD8-conjugating enzyme E2 F, an E2 ubiquitin-like conjugating enzyme that catalyzes neddylation of cullin proteins, regulating cullin-RING ligase (CRL) activity. The UniProt recommended name is NEDD8-conjugating enzyme E2 F (UBE2F). This enzyme cooperates with the NEDD8-activating enzyme (NAE1/UBA3) and specific E3 ligases to attach NEDD8 to target substrates, modulating protein degradation pathways.

Functionally, UBE2F antibody identifies a 185-amino-acid cytoplasmic enzyme that activates and transfers NEDD8 to cullin 5 and other CRL components. UBE2F works specifically with the E3 ligase RBX2 (RNF7), forming a selective neddylation module distinct from UBE2M (UBC12). Through this modification, UBE2F regulates CRL activity, affecting cell cycle progression, signal transduction, and stress responses.

The UBE2F gene is located on chromosome 5q31.2 and is expressed in multiple tissues, including testis, brain, and lung. Its expression is cell cycle-dependent and induced under oxidative stress. UBE2F ensures timely activation of CRLs, thereby influencing ubiquitin-mediated degradation of regulatory proteins such as p27 and HIF1A.

Pathologically, dysregulation of UBE2F contributes to cancer and oxidative stress-related disorders. Overexpression promotes degradation of tumor suppressors, while inhibition can stabilize proteins that restrain cell proliferation. UBE2F has emerged as a therapeutic target for modulating proteostasis and redox balance in malignant cells.

UBE2F antibody is suitable for western blotting, immunoprecipitation, and immunofluorescence to detect neddylation components and study ubiquitin-like modification systems. NSJ Bioreagents provides validated UBE2F antibody reagents for investigating protein turnover, neddylation, and cellular signaling.

Structurally, UBE2F contains a conserved catalytic cysteine residue within its E2 core domain, forming a thioester bond with activated NEDD8. This structure enables transfer to substrate-bound E3 ligases. This antibody helps characterize UBE2F's mechanistic role in the neddylation cascade and its impact on CRL activity.

UniProt: Q969M7