Esterase D anticorps (AA 1-282)

N° du produit ABIN7869044

Cet anticorps Lapin Polyclonal détecte spécifiquement Esterase D dans WB, ELISA, IF, ICC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Esterase D anticorps (AA 1-282) (ABIN7869044)

Antigène: Esterase D (ESD)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Esterase D est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-Esterase D anticorps

Épitope: AA 1-282

Fonction: ESD Antibody / Esterase D

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human ESD recombinant protein (Position: M1-A282) was used as the immunogen for the ESD antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the ESD antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the ESD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur Esterase D

Antigène: Esterase D (ESD)

Autre désignation: ESD

Sujet: ESD antibody detects Esterase D, encoded by the ESD gene on chromosome 13q14.11. ESD antibody is used to study this cytosolic serine hydrolase involved in detoxification and xenobiotic metabolism. ESD was originally characterized as a polymorphic enzyme used in forensic medicine for paternity testing and blood typing. Beyond this historical role, Esterase D has biological functions in processing of ester-containing molecules, detoxification of aldehydes, and metabolism of drugs and environmental chemicals. It is expressed in liver, kidney, lung, and erythrocytes, reflecting its general detoxification role.

Structurally, Esterase D is a 282 amino acid protein with an alpha/beta hydrolase fold. It contains the conserved catalytic triad of serine, histidine, and aspartate typical of serine esterases. Its active site is adapted for hydrolyzing a wide range of small esters. Genetic polymorphisms in ESD lead to enzyme variants with distinct electrophoretic mobility, which historically formed the basis of forensic applications. The conserved structure supports its role as a versatile detoxifying enzyme.

Functionally, ESD hydrolyzes ester bonds in endogenous and exogenous compounds, contributing to clearance of aldehydes and drugs. It also participates in formaldehyde detoxification by metabolizing S-formylglutathione. Knockout or deficiency studies suggest that loss of Esterase D activity impairs detoxification pathways and increases susceptibility to oxidative stress. Researchers employ ESD antibody to study its enzymatic activity, tissue distribution, and regulation under toxicant exposure.

Clinically, ESD deficiency has been associated with retinoblastoma and other developmental disorders due to its chromosomal location near the RB1 tumor suppressor gene. Altered ESD activity is also reported in liver disease, hemolytic anemia, and metabolic disorders. As a polymorphic enzyme, Esterase D has served as a genetic marker in population genetics and forensic investigations. Its ability to detoxify aldehydes and esters makes it relevant to environmental health and pharmacology. NSJ Bioreagents supplies ESD antibody for research into metabolism, toxicology, and disease associations.

Experimentally, ESD antibody is used in western blotting to detect the ~33 kDa protein, in enzymatic assays to measure esterase activity, and in immunohistochemistry to study tissue expression. Immunoprecipitation with ESD antibody allows analysis of its enzyme complexes and potential regulatory partners. These applications provide tools for dissecting detoxification pathways and oxidative stress regulation.

UniProt: P10768