TFDP3 anticorps (AA 1-305)

N° du produit ABIN7869171

L’anticorps anti-TFDP3 Polyclonal Lapin est utilisé pour la détection de TFDP3 dans des échantillons de Humain. Il a été validé pour ELISA, WB, IHC, IF et ICC.

Aperçu rapide pour TFDP3 anticorps (AA 1-305) (ABIN7869171)

Antigène: TFDP3 (Transcription Factor Dp Family, Member 3 (TFDP3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TFDP3 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-TFDP3 anticorps

Épitope: AA 1-305

Fonction: TFDP3 Antibody / Transcription factor Dp family member 3

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human TFDP3 recombinant protein (Position: M1-E305) was used as the immunogen for the TFDP3 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the TFDP3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the TFDP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur TFDP3

Antigène: TFDP3 (Transcription Factor Dp Family, Member 3 (TFDP3))

Autre désignation: TFDP3

Sujet: TFDP3 antibody recognizes Transcription factor Dp family member 3, a unique atypical DP protein that modulates E2F-dependent transcription and cell cycle regulation. Unlike canonical DP proteins (TFDP1 and TFDP2), TFDP3 acts as a transcriptional repressor rather than an activator. It binds to E2F transcription factors but lacks the transactivation domain necessary for promoting gene expression, thereby acting as a competitive inhibitor. The TFDP3 antibody is an essential tool for studying cell proliferation, differentiation, apoptosis, and oncogenic transformation regulated through the E2F/DP pathway.

TFDP3 is encoded by the TFDP3 gene located on human chromosome Xq26.2. The protein consists of a conserved DNA-binding domain and a dimerization region that facilitates complex formation with E2F1. This heterodimerization blocks the transcriptional activation of E2F target genes involved in DNA synthesis and cell cycle progression. As a result, TFDP3 contributes to cell cycle arrest and resistance to apoptosis, especially under stress or DNA damage conditions.

The TFDP3 antibody is used to detect the 45-47 kDa nuclear protein in various cell lines and tissues. Immunofluorescence reveals nuclear localization consistent with its function as a transcriptional regulator. TFDP3 is predominantly expressed in testis, certain stem cells, and a subset of cancer tissues. Its overexpression has been reported in hepatocellular carcinoma, lung adenocarcinoma, and breast cancer, where it promotes survival by suppressing E2F1-induced apoptosis. Mechanistically, TFDP3 interferes with E2F1's ability to induce p53-independent cell death, thereby facilitating oncogenic proliferation.

Beyond cell cycle regulation, TFDP3 participates in differentiation processes and developmental gene expression control. It is also implicated in DNA repair regulation and stress-induced transcriptional reprogramming. Studies have shown that silencing TFDP3 enhances the cytotoxic effects of chemotherapeutic agents, making it a potential therapeutic target. NSJ Bioreagents offers a validated TFDP3 antibody optimized for western blot, immunohistochemistry, and chromatin immunoprecipitation, providing researchers with a robust reagent for dissecting E2F pathway regulation and its role in tumor biology.

UniProt: Q5H9I0

Pathways: Cycle Cellulaire