MCM9 anticorps (AA 1-644)

N° du produit ABIN7869515

Cet anticorps Lapin Polyclonal détecte spécifiquement MCM9 dans WB, ELISA et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour MCM9 anticorps (AA 1-644) (ABIN7869515)

Antigène: MCM9 (Minichromosome Maintenance Deficient 9 (MCM9))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCM9 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-MCM9 anticorps

Épitope: AA 1-644

Fonction: MCM9 Antibody / Minichromosome maintenance complex component 9

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human MCM9 recombinant protein (Position: M1-L644) was used as the immunogen for the MCM9 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the MCM9 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the MCM9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur MCM9

Antigène: MCM9 (Minichromosome Maintenance Deficient 9 (MCM9))

Autre désignation: MCM9

Sujet: MCM9 antibody detects Minichromosome maintenance complex component 9, a helicase-associated protein essential for DNA replication licensing and homologous recombination repair. Encoded by the MCM9 gene on chromosome 6q22.31, this nuclear protein functions as part of the MCM8-MCM9 complex, which assists in DNA replication origin activation and double-strand break repair. MCM9 plays a critical role in maintaining genomic stability and facilitating proper DNA synthesis during S phase.

Structurally, MCM9 is a 1,143-amino-acid nuclear protein of approximately 130 kilodaltons containing an AAA+ ATPase domain typical of MCM family helicases. Although it lacks intrinsic helicase activity, MCM9 interacts with MCM8 to form a functional complex that promotes DNA end resection and strand invasion during homologous recombination. It also associates with replication proteins such as RPA and RAD51, coordinating DNA repair with replication fork progression.

The MCM9 antibody is widely used in cell cycle, DNA repair, and cancer research to study replication licensing, recombination mechanisms, and genome maintenance. Western blot analysis detects a 130 kilodalton band corresponding to MCM9, while immunofluorescence shows distinct nuclear foci that colocalize with replication and repair markers. This antibody enables detailed analysis of DNA replication and damage response pathways.

Functionally, MCM9 is required for the recruitment of repair proteins to sites of DNA damage and for the restart of stalled replication forks. Mutations in MCM9 are associated with hereditary colorectal cancer syndromes and premature ovarian failure due to impaired DNA repair capacity. Loss of MCM9 function results in genomic instability and sensitivity to DNA-damaging agents. The MCM9 antibody provides a key reagent for investigating replication integrity, homologous recombination, and tumor suppression mechanisms. NSJ Bioreagents validates this antibody for its applications, ensuring accurate and reproducible detection for genome stability studies.

UniProt: Q9NXL9