MAML1 anticorps (AA 1-998)

N° du produit ABIN7869672

L’anticorps anti-MAML1 Polyclonal Lapin est utilisé pour la détection de MAML1 dans des échantillons de Humain. Il a été validé pour ELISA, WB, FACS et IF.

Aperçu rapide pour MAML1 anticorps (AA 1-998) (ABIN7869672)

Antigène: MAML1 (Mastermind-Like 1 (MAML1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAML1 est non-conjugé

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Détail du produit anti-MAML1 anticorps

Épitope: AA 1-998

Fonction: MAML1 Antibody / Mastermind-like protein 1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human MAML1 recombinant protein (Position: M1-K998) was used as the immunogen for the MAML1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the MAML1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the MAML1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur MAML1

Antigène: MAML1 (Mastermind-Like 1 (MAML1))

Autre désignation: MAML1

Sujet: MAML1 antibody detects Mastermind-like protein 1, a transcriptional coactivator that plays a key role in the Notch signaling pathway, which regulates cell fate determination, differentiation, and proliferation. Encoded by the MAML1 gene on chromosome 5q35.3, this nuclear protein acts as a core component of the Notch transcriptional activation complex. Upon Notch receptor activation and nuclear translocation of the Notch intracellular domain (NICD), MAML1 binds NICD and the transcription factor CSL (CBF1/RBPJ), forming a ternary complex that recruits additional coactivators to initiate target gene transcription.

MAML1 contains an N-terminal basic domain responsible for binding NICD and CSL, a central acidic transcriptional activation domain, and a C-terminal domain that mediates interactions with additional transcription factors and coactivators such as p300/CBP. Through these interactions, MAML1 promotes transcription of Notch target genes including HES1, HEY1, and MYC, which are involved in cell differentiation and proliferation. Because Notch signaling is crucial for tissue development and homeostasis, MAML1 serves as a master regulator of developmental pathways across multiple cell types, including neural, hematopoietic, and epithelial lineages.

The MAML1 antibody is widely used in molecular biology, developmental biology, and cancer research to assess Notch pathway activation and transcriptional regulation. Western blot analysis typically identifies a 115 kilodalton band corresponding to MAML1, while immunofluorescence reveals nuclear localization that overlaps with Notch pathway components. This antibody enables the study of transcriptional coactivation events and gene regulation in Notch-mediated signaling contexts.

Beyond Notch signaling, MAML1 also functions as a coactivator for other transcriptional regulators such as MEF2C, p53, and beta-catenin, broadening its impact on gene networks related to cell growth and apoptosis. Dysregulation of MAML1 has been linked to oncogenic transformation, particularly in leukemia, glioma, and breast cancer, where aberrant Notch signaling drives uncontrolled proliferation. The MAML1 antibody provides a reliable tool for investigating transcriptional complexes and signaling crosstalk mechanisms. NSJ Bioreagents offers this antibody validated for its applications, ensuring reproducibility and specificity for transcriptional and signaling studies.

UniProt: Q92585

Pathways: Signalisation Notch, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development