AQP2 anticorps (AA 102-271)

N° du produit ABIN7869912

L’anticorps anti-AQP2 Polyclonal Lapin est utilisé pour la détection de AQP2 dans des échantillons de Rat et Souris. Il a été validé pour WB, ELISA et FACS.

Aperçu rapide pour AQP2 anticorps (AA 102-271) (ABIN7869912)

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivité: Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AQP2 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-AQP2 anticorps

Épitope: AA 102-271

Fonction: Aqp2 Antibody / Aquaporin 2

Purification: Immunogen affinity purified

Immunogène: E.coli-derived mouse Aqp2 recombinant protein (Position: A102-A271) was used as the immunogen for the Aqp2 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Aqp2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the Aqp2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur AQP2

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Autre désignation: Aqp2

Sujet: The AQP2 antibody targets Aquaporin-2, a water channel protein encoded by the AQP2 gene. Aquaporin-2 is an essential component of the kidney's water reabsorption system, located in the apical membrane of collecting duct principal cells. It mediates vasopressin-regulated water transport, thereby controlling urine concentration and maintaining body fluid balance. The AQP2 antibody enables researchers to study renal physiology, osmoregulation, and disorders of water homeostasis such as diabetes insipidus.

Aquaporin-2 belongs to the aquaporin family of integral membrane proteins that selectively transport water across biological membranes. Under the influence of the antidiuretic hormone vasopressin, AQP2-containing vesicles are trafficked to and fused with the apical plasma membrane, increasing water permeability. The AQP2 antibody provides a reliable means to visualize this regulated translocation process, making it a key tool for examining vasopressin signaling and collecting duct responsiveness.

Mutations in the AQP2 gene lead to nephrogenic diabetes insipidus (NDI), characterized by excessive urine production and an inability to concentrate urine despite normal vasopressin levels. The AQP2 antibody supports research into this condition by enabling detection of defective or mislocalized Aquaporin-2 in renal tissue. Experimental models show that AQP2 deficiency disrupts water retention and contributes to dehydration, underscoring its clinical relevance in renal water management.

Beyond kidney physiology, Aquaporin-2 has been detected in reproductive tissues and the central nervous system, suggesting broader roles in fluid transport and homeostasis. The AQP2 antibody supports such investigations, allowing comparative expression analysis across tissues and experimental conditions. Regulation of AQP2 expression and trafficking involves multiple pathways including cyclic AMP, protein kinase A, and ubiquitination-dependent endocytosis.

The AQP2 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, producing distinct apical membrane staining in kidney tissue. NSJ Bioreagents provides this antibody with verified specificity and reproducibility for renal physiology, endocrinology, and molecular signaling research. By enabling precise detection of Aquaporin-2, the AQP2 antibody advances understanding of water transport regulation and the molecular mechanisms underlying disorders of water balance.

UniProt: P56402

Pathways: Response to Water Deprivation