Espin anticorps (AA 143-854)

N° du produit ABIN7870704

L’anticorps anti-Espin Polyclonal Lapin est utilisé pour la détection de Espin dans des échantillons de Souris et Rat. Il a été validé pour WB et ELISA.

Aperçu rapide pour Espin anticorps (AA 143-854) (ABIN7870704)

Antigène: Espin (ESPN)

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Espin est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-Espin anticorps

Épitope: AA 143-854

Fonction: ESPN Antibody / Espin

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human ESPN recombinant protein (Position: H143-Y854) was used as the immunogen for the ESPN antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the ESPN antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the ESPN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur Espin

Antigène: Espin (ESPN)

Autre désignation: ESPN

Sujet: ESPN antibody detects Espin, an actin-binding protein essential for the assembly and maintenance of parallel actin bundles in sensory cells, particularly in the stereocilia of hair cells in the inner ear. ESPN is required for hearing and balance by supporting the structural integrity of mechanosensory projections. The ESPN antibody is widely used in auditory biology, cytoskeletal, and sensory neuroscience research to study actin organization, hair cell morphology, and mechanotransduction.

ESPN is encoded by the ESPN gene located on human chromosome 1p36.31. The protein exists in multiple isoforms ranging from 1,000 to 1,100 amino acids, each containing actin-binding and proline-rich domains that mediate crosslinking of actin filaments. ESPN localizes to microvillar and stereociliary actin cores, where it provides structural reinforcement and length regulation.

The ESPN antibody detects bands between 90 and 110 kilodaltons depending on isoform and shows intense labeling of stereocilia and microvilli under immunofluorescence microscopy. ESPN promotes actin bundle stability by counteracting depolymerizing factors such as cofilin, ensuring the long-term maintenance of hair cell stereocilia. Its overexpression induces elongation of actin protrusions, while loss of function causes disorganization and degeneration of stereocilia, leading to deafness.

Mutations in ESPN are responsible for autosomal recessive deafness and vestibular dysfunction in both humans and animal models. Beyond auditory tissue, ESPN contributes to brush border maintenance in epithelial cells and to filopodia dynamics in neurons and immune cells, reflecting its broad cytoskeletal role.

Because of its crucial function in actin filament assembly and mechanosensory integrity, ESPN serves as a vital biomarker for studies of hearing, balance, and cytoskeletal structure. NSJ Bioreagents provides a validated ESPN antibody optimized for its applications, supporting investigations into actin regulation, auditory biology, and cellular architecture.

UniProt: B1AK53

Pathways: Sensory Perception of Sound