TMEM9 anticorps (AA 18-183)

N° du produit ABIN7871397

L’anticorps anti-TMEM9 Polyclonal Lapin est utilisé pour la détection de TMEM9 dans des échantillons de Humain, Souris et Rat. Il a été validé pour ELISA, WB et FACS.

Aperçu rapide pour TMEM9 anticorps (AA 18-183) (ABIN7871397)

Antigène: TMEM9 (Transmembrane Protein 9 (TMEM9))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TMEM9 est non-conjugé

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-TMEM9 anticorps

Épitope: AA 18-183

Fonction: TMEM9 Antibody / Transmembrane protein 9

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human TMEM9 recombinant protein (Position: A18-S183) was used as the immunogen for the TMEM9 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the TMEM9 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the TMEM9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur TMEM9

Antigène: TMEM9 (Transmembrane Protein 9 (TMEM9))

Autre désignation: TMEM9

Sujet: TMEM9 antibody detects Transmembrane protein 9, encoded by the TMEM9 gene on chromosome 1q41. TMEM9 antibody is widely used in research on vesicle trafficking, lysosomal biology, and cancer. TMEM9 is a small lysosomal transmembrane protein that functions as a regulator of vesicle acidification and lysosomal activity. It contributes to autophagy, protein degradation, and receptor recycling, making it important for cell metabolism and signaling.

Structurally, TMEM9 is a ~21 kDa protein predicted to contain multiple transmembrane domains. It localizes to late endosomes and lysosomes, where it interacts with vacuolar H+-ATPases (V-ATPases) to promote acidification of the organelle lumen. This acidification is required for hydrolytic enzyme activity, autophagosome maturation, and lysosomal degradation. TMEM9 is conserved across vertebrates and is essential for maintaining lysosomal homeostasis.

Functionally, TMEM9 promotes lysosomal degradation and autophagy by enhancing V-ATPase assembly. It also regulates receptor recycling and endosomal trafficking. Dysregulation of TMEM9 disrupts lysosomal activity, leading to accumulation of undegraded proteins and impaired autophagy. Researchers use TMEM9 antibody to study vesicle trafficking, lysosomal acidification, and autophagy regulation.

Clinically, TMEM9 has been linked to cancer progression. Overexpression of TMEM9 has been reported in colorectal and liver cancers, where it promotes tumor growth by enhancing Wnt/beta-catenin signaling and metabolic adaptation. It may serve as a biomarker of aggressive tumor behavior and a potential therapeutic target. NSJ Bioreagents provides TMEM9 antibody for lysosomal, cancer, and metabolism studies.

Experimentally, TMEM9 antibody is applied in western blotting to detect the ~21 kDa protein, in immunofluorescence to study lysosomal localization, and in immunohistochemistry to assess tissue-specific expression. Co-immunoprecipitation with TMEM9 antibody helps identify interactions with V-ATPase components.

UniProt: Q9P0T7