MYOD1 anticorps (AA 180-189)

N° du produit ABIN7871437

Cet anticorps anti-MYOD1 Monoclonal Souris (Clone SPM427) (ABIN7871437) détecte spécifiquement MYOD1 dans IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour MYOD1 anticorps (AA 180-189) (ABIN7871437)

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MYOD1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM427

Détail du produit anti-MYOD1 anticorps

Épitope: AA 180-189

Fonction: Anti-MyoD1 Antibody

Purification: Protein G affinity chromatography

Immunogène: Recombinant mouse protein was used as the immunogen for this anti-MyoD1 antibody. The epitope of this mAb maps between amino acid 180-189.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the anti-MyoD1 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-MyoD1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur MYOD1

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: MyoD1

Sujet: Recognizes a phosphor-protein of 45 kDa, identified as MyoD1. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development