SYCN anticorps (AA 19-134)

N° du produit ABIN7871559

L’anticorps anti-SYCN Polyclonal Lapin est utilisé pour la détection de SYCN dans des échantillons de Humain, Souris et Rat. Il a été validé pour ELISA, WB, ICC, FACS et IF.

Aperçu rapide pour SYCN anticorps (AA 19-134) (ABIN7871559)

Antigène: SYCN (Syncollin (SYCN))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SYCN est non-conjugé

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Détail du produit anti-SYCN anticorps

Épitope: AA 19-134

Fonction: SYCN Antibody / Syncollin

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human SYCN recombinant protein (Position: A19-S134) was used as the immunogen for the SYCN antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the SYCN antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the SYCN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur SYCN

Antigène: SYCN (Syncollin (SYCN))

Autre désignation: SYCN

Sujet: SYCN antibody detects Syncollin, a secretory vesicle membrane protein that plays an important role in pancreatic enzyme secretion and zymogen granule exocytosis. Encoded by the SYCN gene on chromosome 19q13.33, Syncollin is a small protein localized to the membranes of secretory vesicles within pancreatic acinar cells and other exocrine tissues. It functions as an accessory factor that promotes granule maturation, docking, and fusion with the plasma membrane during regulated secretion.

Structurally, Syncollin contains a coiled-coil domain responsible for oligomerization and membrane tethering, allowing it to form homo-oligomeric complexes on the luminal surface of zymogen granules. These complexes interact with SNARE proteins and other components of the exocytic machinery to facilitate vesicle fusion and enzyme release. SYCN contributes to the organization of zymogen granule membranes and ensures precise regulation of digestive enzyme secretion in response to cholecystokinin and other secretagogues.

The SYCN antibody is widely used in cell biology, gastrointestinal physiology, and secretion research to investigate vesicle dynamics, exocytosis, and pancreatic function. Western blot analysis detects a 13 kilodalton band corresponding to Syncollin, while immunofluorescence reveals punctate staining in the apical cytoplasm of acinar cells and along the zymogen granule membranes. This antibody supports analysis of regulated secretory pathways and granule maturation in exocrine tissues.

Disruption or reduced expression of SYCN impairs vesicle exocytosis and digestive enzyme secretion, leading to altered pancreatic physiology. In experimental models, loss of Syncollin function results in defective granule docking and diminished amylase release, highlighting its essential role in the secretory machinery. The SYCN antibody provides a robust reagent for examining vesicle organization, exocytotic mechanisms, and the structural biology of secretory organelles. NSJ Bioreagents validates this antibody for its applications to ensure reliable and reproducible results for studies on secretion and exocrine function.

UniProt: Q0VAF6