PDE8A anticorps (AA 32-495)

N° du produit ABIN7873925

Cet anticorps Lapin Polyclonal détecte spécifiquement PDE8A dans WB, ELISA et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour PDE8A anticorps (AA 32-495) (ABIN7873925)

Antigène: PDE8A (phosphodiesterase 8A (PDE8A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDE8A est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-PDE8A anticorps

Épitope: AA 32-495

Fonction: PDE8A Antibody / Phosphodiesterase 8A

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human PDE8A recombinant protein (Position: R32-E495) was used as the immunogen for the PDE8A antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PDE8A antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PDE8A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur PDE8A

Antigène: PDE8A (phosphodiesterase 8A (PDE8A))

Autre désignation: PDE8A

Sujet: PDE8A antibody detects Phosphodiesterase 8A, an enzyme that specifically hydrolyzes cyclic adenosine monophosphate (cAMP) to AMP, thereby regulating intracellular signaling pathways mediated by this second messenger. PDE8A belongs to the type 8 family of cyclic nucleotide phosphodiesterases, which are distinct in their insensitivity to common PDE inhibitors such as IBMX. The PDE8A antibody is widely used in studies of hormonal regulation, metabolism, and immune signaling, where precise modulation of cAMP levels is critical.

PDE8A is encoded by the PDE8A gene located on human chromosome 15q25.3. The enzyme is approximately 93 kilodaltons and contains a conserved catalytic domain flanked by unique N-terminal and C-terminal regulatory regions. PDE8A is expressed in various tissues, including adrenal gland, heart, testis, and immune cells, where it contributes to compartmentalized cAMP degradation and pathway specificity. Its high substrate affinity and resistance to standard inhibitors make it a key regulator of localized signaling domains.

The PDE8A antibody detects multiple isoforms ranging from 85-100 kilodaltons on western blot and reveals cytoplasmic and perinuclear staining in immunofluorescence assays. PDE8A associates with A-kinase anchoring proteins (AKAPs) and participates in cAMP microdomain organization, influencing downstream activation of protein kinase A (PKA) and exchange proteins activated by cAMP (EPACs). In immune cells, PDE8A modulates T-cell receptor signaling and cytokine production, contributing to immune tolerance and inflammatory regulation.

Functional studies indicate that PDE8A influences steroidogenesis, insulin secretion, and energy balance by controlling cAMP-mediated transcriptional events. Knockdown or pharmacological inhibition enhances cAMP signaling and affects processes such as sperm motility, cortisol synthesis, and cardiac contractility. Altered PDE8A expression has been linked to autoimmune diseases, infertility, and metabolic disorders.

PDE8A also plays roles in cancer biology, where its dysregulation affects cell proliferation and migration through altered PKA activity and transcriptional responses. NSJ Bioreagents provides a validated PDE8A antibody optimized for western blot, flow cytometry, and enzyme localization assays, enabling precise exploration of cyclic nucleotide signaling, endocrine regulation, and immune modulation.

UniProt: O60658

Pathways: cAMP Metabolic Process