LMO2 anticorps (AA 4-150)

N° du produit ABIN7874657

Cet anticorps Lapin Polyclonal détecte spécifiquement LMO2 dans WB, FACS, ELISA et IP. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour LMO2 anticorps (AA 4-150) (ABIN7874657)

Antigène: LMO2 (LIM Domain Only 2 (Rhombotin-Like 1) (LMO2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LMO2 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP)

Détail du produit anti-LMO2 anticorps

Épitope: AA 4-150

Fonction: LMO2 Antibody / Rhombotin 2

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human LMO2 recombinant protein (Position: A4-E150) was used as the immunogen for the LMO2 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the LMO2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the LMO2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur LMO2

Antigène: LMO2 (LIM Domain Only 2 (Rhombotin-Like 1) (LMO2))

Autre désignation: LMO2

Sujet: LMO2 antibody detects LIM domain only protein 2, encoded by the LMO2 gene on chromosome 11p13. LMO2 antibody is commonly applied in research on hematopoiesis, transcriptional regulation, and cancer biology. LMO2 is a transcriptional co-regulator without intrinsic DNA-binding activity, instead, it functions as an adaptor protein that brings together DNA-binding transcription factors within multiprotein complexes. It plays essential roles in hematopoietic stem cell maintenance, vascular development, and T-cell differentiation. Expression is high in hematopoietic progenitors and endothelial cells, but tightly regulated, as dysregulation promotes oncogenesis.

Structurally, LMO2 is a small nuclear protein of ~158 amino acids containing two tandem LIM domains, zinc-binding motifs that mediate protein-protein interactions. These LIM domains allow LMO2 to interact with basic helix-loop-helix (bHLH) proteins such as TAL1, GATA1, and LDB1, forming complexes that regulate transcription of genes required for hematopoietic development. Alternative splicing generates isoforms with distinct interaction patterns.

Functionally, LMO2 acts as a bridging factor, linking transcription factors to DNA regulatory regions. It is essential for erythropoiesis and angiogenesis, controlling expression of genes involved in cell fate decisions. Knockout mice lacking LMO2 exhibit embryonic lethality due to failure of blood vessel and hematopoietic development. In hematopoietic stem cells, LMO2 ensures long-term maintenance and self-renewal. Researchers use LMO2 antibody to study transcriptional regulation in blood formation and cancer biology.

Clinically, LMO2 is a well-known oncogene in T-cell acute lymphoblastic leukemia (T-ALL), where aberrant expression drives leukemogenesis. Chromosomal translocations or retroviral insertional mutagenesis can activate LMO2, promoting proliferation of immature T-cells. It also has roles in diffuse large B-cell lymphoma and other hematologic malignancies. Conversely, in normal physiology, its expression serves as a marker for angiogenesis and hematopoietic stem cell activity. NSJ Bioreagents provides LMO2 antibody to support oncology and stem cell research.

Experimentally, LMO2 antibody is used in western blotting to detect the ~18 kDa protein, in immunohistochemistry to examine expression in hematopoietic and endothelial tissues, and in immunofluorescence microscopy to confirm nuclear localization. Co-immunoprecipitation with LMO2 antibody allows identification of transcriptional complexes and regulatory partners.

UniProt: P25791

Pathways: Chromatin Binding