Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 49-1456) anticorps

N° du produit ABIN7875240

L’anticorps anti-Regulating Synaptic Membrane Exocytosis 1 Polyclonal Lapin est utilisé pour la détection de Regulating Synaptic Membrane Exocytosis 1 dans des échantillons de Rat et Souris. Il a été validé pour WB, ELISA et IHC.

Aperçu rapide pour Regulating Synaptic Membrane Exocytosis 1 (RIMS1) (AA 49-1456) anticorps (ABIN7875240)

Antigène: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Reactivité: Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit

Épitope: AA 49-1456

Fonction: RIMS1 Antibody / Regulating synaptic membrane exocytosis protein 1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human RIM1/RIMS1 recombinant protein (Position: K49-E1456) was used as the immunogen for the RIMS1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the RIMS1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the RIMS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détail du antigène

Antigène: Regulating Synaptic Membrane Exocytosis 1 (RIMS1)

Autre désignation: RIMS1

Sujet: RIMS1 antibody detects Regulating synaptic membrane exocytosis protein 1, a presynaptic scaffold protein essential for synaptic vesicle priming and neurotransmitter release. Encoded by the RIMS1 gene on chromosome 6q13, this protein belongs to the RIM (Regulating synaptic membrane exocytosis) family and functions as a central organizer of the active zone at presynaptic terminals. RIMS1 coordinates synaptic vesicle docking with calcium-triggered exocytosis by linking voltage-gated calcium channels to synaptic vesicle proteins and cytoskeletal components.

Structurally, RIMS1 contains zinc finger, PDZ, C2A, and C2B domains that mediate interactions with Munc13, RAB3A, ELKS, liprins, and calcium channels. Through these interactions, RIMS1 facilitates vesicle tethering, calcium channel clustering, and efficient neurotransmitter release. It localizes to the cytoplasmic face of active zones in excitatory and inhibitory neurons, where it serves as a molecular hub integrating presynaptic signaling and vesicle dynamics.

The RIMS1 antibody is used in neurobiology and synaptic physiology research to study synaptic vesicle trafficking, neurotransmitter release, and presynaptic organization. Western blot analysis typically identifies a 175-180 kilodalton band corresponding to RIMS1, while immunofluorescence reveals punctate staining at presynaptic terminals. This antibody supports studies of neuronal communication, synaptic plasticity, and calcium-dependent exocytosis.

Mutations in RIMS1 are associated with cone-rod dystrophy and autism spectrum disorders, highlighting its importance in both neuronal development and sensory transmission. RIMS1 deficiency disrupts synaptic organization and impairs neurotransmitter release efficiency. The RIMS1 antibody is a key reagent for analyzing synaptic machinery and presynaptic protein complexes. NSJ Bioreagents validates this antibody for western blotting and immunohistochemistry, ensuring reliable detection in neuronal and synaptic research.

UniProt: Q86UR5

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity