LRRC75A/FAM211A anticorps (AA 52-292)

N° du produit ABIN7875558

L’anticorps anti-LRRC75A/FAM211A Polyclonal Lapin est utilisé pour la détection de LRRC75A/FAM211A dans des échantillons de Humain. Il a été validé pour WB, ELISA et FACS.

Aperçu rapide pour LRRC75A/FAM211A anticorps (AA 52-292) (ABIN7875558)

Antigène: LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LRRC75A/FAM211A est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-LRRC75A/FAM211A anticorps

Épitope: AA 52-292

Fonction: LRRC75A Antibody / FAM211A

Purification: Antigen affinity purified

Immunogène: An E.coli-derived human recombinant protein (amino acids H52-Q292) was used as the immunogen for the LRRC75A antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the LRRC75A antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the LRRC75A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur LRRC75A/FAM211A

Antigène: LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))

Autre désignation: LRRC75A

Sujet: Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-?B pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.

UniProt: Q8NAA5