LRRC75A/FAM211A anticorps (AA 52-292) (Cy3)

N° du produit ABIN8005557

L’anticorps anti-LRRC75A/FAM211A Polyclonal Lapin est utilisé pour la détection de LRRC75A/FAM211A dans des échantillons de Humain. Il a été validé pour FACS.

Aperçu rapide pour LRRC75A/FAM211A anticorps (AA 52-292) (Cy3) (ABIN8005557)

Antigène: LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LRRC75A/FAM211A est conjugé à/à la Cy3

Application: Flow Cytometry (FACS)

Détail du produit anti-LRRC75A/FAM211A anticorps (Cy3)

Épitope: AA 52-292

Fonction: Anti-LRRC75A Antibody Cy3 Conjugated

Réactivité croisée (Details): No cross reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human LRRC75A recombinant protein (Position: H52-Q292).

Isotype: IgG

Information d'application

Indications d'application: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Date de péremption: 12 months

Détails sur LRRC75A/FAM211A

Antigène: LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))

Autre désignation: LRRC75A

Sujet:

Background: Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-κB pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.

Gene Full Name: leucine rich repeat containing 75A

ID gène: 388341

UniProt: Q8NAA5