CDCA8 anticorps (AA 72-280)

N° du produit ABIN7876633

Cet anticorps anti-CDCA8 Polyclonal Lapin (ABIN7876633) détecte spécifiquement CDCA8 dans WB, ELISA, FACS, IF et ICC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CDCA8 anticorps (AA 72-280) (ABIN7876633)

Antigène: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDCA8 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-CDCA8 anticorps

Épitope: AA 72-280

Fonction: CDCA8 Antibody / Cell division cycle-associated protein 8 / Borealin

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human CDCA8 recombinant protein (Position: D72-K280) was used as the immunogen for the CDCA8 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the CDCA8 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the CDCA8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur CDCA8

Antigène: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Autre désignation: CDCA8

Sujet: CDCA8 antibody recognizes Cell division cycle-associated protein 8, also known as Borealin, a core component of the chromosomal passenger complex (CPC) that governs chromosome segregation, spindle assembly, and cytokinesis. Encoded by the CDCA8 gene on chromosome 1p34.3, this protein interacts with Aurora B kinase, INCENP, and Survivin to form a functional complex that ensures accurate mitotic progression. Borealin is essential for proper kinetochore-microtubule attachment, spindle checkpoint activation, and correction of mitotic errors, thereby safeguarding genomic stability during cell division.

During mitosis, Borealin localizes to centromeres in metaphase and relocates to the spindle midzone during anaphase, coordinating the transition from chromosome alignment to cytokinesis. Loss of CDCA8 function results in chromosome missegregation, lagging chromatids, and multinucleation, phenotypes commonly associated with aneuploidy and cancer. Expression of CDCA8 peaks during the G2/M phase, reflecting its cell cycle-dependent regulation. Overexpression has been documented in a range of malignancies including breast, lung, and colorectal cancers, correlating with poor prognosis and high proliferative indices.

The CDCA8 antibody is widely used in cell cycle and cancer research to detect Borealin localization and expression dynamics. Immunofluorescence analysis with this antibody reveals distinct centromeric staining during metaphase and midbody localization in late mitosis. Western blotting identifies a 35 kilodalton band corresponding to the full-length protein. Because the chromosomal passenger complex regulates essential mitotic checkpoints, the CDCA8 antibody provides a critical tool for studying mechanisms of mitotic fidelity and tumorigenesis.

CDCA8 cooperates with Aurora B to phosphorylate substrates involved in spindle tension sensing and cytokinesis. Disruption of this interaction impairs CPC activity and leads to mitotic failure. Borealin's ability to bind DNA through its N-terminal domain and oligomerize via its coiled-coil region enables structural stability of the CPC. Inhibition or knockdown of CDCA8 sensitizes tumor cells to microtubule-targeting drugs, highlighting its potential as a therapeutic target. The antibody thus supports translational research exploring CPC inhibitors and mitotic checkpoint modulators in cancer therapy.

In developmental biology, CDCA8 is required for proper cell division during embryogenesis and organogenesis. The CDCA8 antibody facilitates identification of proliferating cells in rapidly dividing tissues and embryonic stem cell cultures. NSJ Bioreagents provides this antibody validated for its applications, ensuring consistent results in mitotic and oncogenic studies.

UniProt: Q53HL2

Pathways: Cycle Cellulaire, M Phase