INTS4 anticorps (AA 8-963)

N° du produit ABIN7876850

Cet anticorps Lapin Polyclonal détecte spécifiquement INTS4 dans WB, ELISA, IHC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour INTS4 anticorps (AA 8-963) (ABIN7876850)

Antigène: INTS4 (Integrator Complex Subunit 4 (INTS4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp INTS4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-INTS4 anticorps

Épitope: AA 8-963

Fonction: INTS4 Antibody / Integrator complex subunit 4

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human INTS4 recombinant protein (Position: R8-R963) was used as the immunogen for the INTS4 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the INTS4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the INTS4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur INTS4

Antigène: INTS4 (Integrator Complex Subunit 4 (INTS4))

Autre désignation: INTS4

Sujet: INTS4 antibody detects Integrator complex subunit 4, encoded by the INTS4 gene on chromosome 11p15.5. INTS4 antibody is widely used in studies of RNA processing, transcriptional regulation, and nuclear complexes. INTS4 is part of the Integrator complex, a multiprotein assembly that associates with RNA polymerase II and is essential for small nuclear RNA (snRNA) 3' end processing. By regulating snRNA maturation, INTS4 contributes to spliceosome function and overall gene expression control.

Structurally, INTS4 is a ~100 kDa protein that interacts with multiple Integrator subunits to stabilize complex assembly. It lacks obvious enzymatic domains but acts as a scaffold, organizing catalytic and RNA-binding subunits. Isoforms generated by alternative splicing may modify complex architecture or activity in specific tissues. Nuclear localization signals target INTS4 to sites of transcription and RNA processing.

Functionally, INTS4 coordinates snRNA 3' end formation, an essential step for spliceosome assembly and pre-mRNA splicing. It also contributes to regulation of enhancer RNA transcription and termination of certain protein-coding genes. Knockdown of INTS4 impairs Integrator stability, leading to widespread RNA processing defects. Researchers use INTS4 antibody to study transcriptional regulation, RNA biology, and spliceosome assembly.

Clinically, INTS4 mutations are associated with neurodevelopmental disorders, where defective RNA processing alters gene expression programs. Dysregulation of Integrator components has been implicated in cancer, particularly in transcription-driven tumors, where abnormal termination enhances oncogene expression. NSJ Bioreagents provides INTS4 antibody for studies of RNA processing, transcriptional regulation, and disease mechanisms.

Experimentally, INTS4 antibody is used in western blotting to detect the ~100 kDa protein, in immunoprecipitation to analyze Integrator complex composition, and in immunofluorescence microscopy to study nuclear localization. Chromatin immunoprecipitation with INTS4 antibody allows analysis of Integrator recruitment to active genes.

UniProt: Q96HW7

Pathways: SARS-CoV-2 Protein Interactome