ube3a anticorps (AA 836-865)

N° du produit ABIN7876950

L’anticorps anti-ube3a Polyclonal Lapin est utilisé pour la détection de ube3a dans des échantillons de Humain. Il a été validé pour WB, IF, FACS et IHC (p).

Aperçu rapide pour ube3a anticorps (AA 836-865) (ABIN7876950)

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ube3a est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ube3a anticorps

Épitope: AA 836-865

Fonction: Ubiquitin-protein ligase E3A Antibody / UBE3A

Purification: Purified

Immunogène: A portion of amino acids 836-865 from the human protein was used as the immunogen for the Ubiquitin-protein ligase E3A antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the Ubiquitin-protein ligase E3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the Ubiquitin-protein ligase E3A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur ube3a

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Autre désignation: Ubiquitin-protein ligase E3A

Sujet: UBE3A interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein. UBE3A binds UBQLN1 and UBQLN2. Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830], also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove. UBE3A contains 1 HECT-type E3 ubiquitin-protein ligase domain.

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway