SHMT2 anticorps (AA 9-504)

N° du produit ABIN7877091

L’anticorps anti-SHMT2 Polyclonal Lapin est utilisé pour la détection de SHMT2 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IHC, IF, ELISA, IP, ICC et FACS.

Aperçu rapide pour SHMT2 anticorps (AA 9-504) (ABIN7877091)

Antigène: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SHMT2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-SHMT2 anticorps

Épitope: AA 9-504

Fonction: SHMT2 Antibody / Serine hydroxymethyltransferase

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human SHMT2 recombinant protein (Position: A9-H504) was used as the immunogen for the SHMT2 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the SHMT2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the SHMT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur SHMT2

Antigène: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Autre désignation: SHMT2

Sujet: The SHMT2 antibody targets Serine hydroxymethyltransferase, mitochondrial, an enzyme encoded by the SHMT2 gene that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate. This reaction is central to one-carbon metabolism, providing key intermediates for nucleotide synthesis, methylation reactions, and redox regulation. Serine hydroxymethyltransferase, mitochondrial operates within the mitochondrial folate cycle and contributes to biosynthetic and energetic demands in proliferating cells. The SHMT2 antibody provides a reliable reagent for studying one-carbon metabolism, mitochondrial function, and cancer metabolism.

Serine hydroxymethyltransferase, mitochondrial forms homotetramers that utilize pyridoxal phosphate (PLP) as a cofactor for catalysis. Its activity links amino acid metabolism with folate-mediated one-carbon flux, coordinating mitochondrial and cytosolic metabolic networks. The SHMT2 antibody enables visualization of this enzyme within mitochondria, supporting studies of its spatial regulation and contribution to biosynthetic pathways under nutrient limitation or hypoxic conditions. SHMT2 expression increases in rapidly dividing cells to sustain nucleotide production and redox balance.

Aberrant activation of one-carbon metabolism, including SHMT2 upregulation, is a hallmark of cancer metabolism. Overexpression of Serine hydroxymethyltransferase, mitochondrial enhances proliferation and resistance to oxidative stress by supplying reduced folate cofactors and NADPH. The SHMT2 antibody facilitates detection of this metabolic enzyme in tumor tissues and cell lines, aiding evaluation of its role in tumorigenesis and metabolic adaptation. SHMT2 has been proposed as a potential therapeutic target, as inhibition disrupts nucleotide synthesis and redox homeostasis in cancer cells.

Beyond oncology, Serine hydroxymethyltransferase, mitochondrial contributes to neural development, mitochondrial respiration, and cellular methylation capacity. It provides one-carbon units necessary for formate generation and DNA methylation reactions that influence epigenetic regulation. The SHMT2 antibody supports research into these metabolic control points, enabling functional analysis of folate cycle dynamics in metabolic disorders, neurodegenerative diseases, and aging.

The SHMT2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing clear mitochondrial staining patterns. NSJ Bioreagents provides this antibody with validated specificity for consistent performance in biochemistry, metabolism, and cancer biology research. By enabling detailed study of Serine hydroxymethyltransferase, mitochondrial expression and activity, the SHMT2 antibody supports discovery into one-carbon metabolism and mitochondrial regulation under physiological and pathological conditions.

UniProt: P34897