WEE1 anticorps (AA 96-130)

N° du produit ABIN7877241

L’anticorps anti-WEE1 Polyclonal Lapin est utilisé pour la détection de WEE1 dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour WEE1 anticorps (AA 96-130) (ABIN7877241)

Antigène: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WEE1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-WEE1 anticorps

Épitope: AA 96-130

Fonction: Wee1 Antibody

Purification: Antigen affinity

Immunogène: A portion of amino acids 96-130 from human Wee1 was used as the immunogen for the Wee1 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the Wee1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the Wee1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur WEE1

Antigène: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Autre désignation: Wee1

Sujet: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. [UniProt]

UniProt: P30291

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, M Phase