CRH anticorps (C-Term)

N° du produit ABIN7877355

L’anticorps anti-CRH Polyclonal Lapin est utilisé pour la détection de CRH dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB et FACS.

Aperçu rapide pour CRH anticorps (C-Term) (ABIN7877355)

Antigène: CRH (Corticotropin Releasing Hormone (CRH))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CRH est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-CRH anticorps

Épitope: C-Term

Fonction: CRH Antibody / Corticotropin Releasing Hormone

Purification: Immunogen affinity purified

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human CRF/CRH was used as the immunogen for the CRH antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the CRH antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the CRH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur CRH

Antigène: CRH (Corticotropin Releasing Hormone (CRH))

Autre désignation: CRH

Sujet: CRH antibody detects Corticotropin releasing hormone, a neuropeptide hormone that regulates the hypothalamic-pituitary-adrenal (HPA) axis and stress response. The UniProt recommended name is Corticotropin releasing hormone (CRH). This 41-amino-acid peptide is synthesized in the hypothalamus and secreted into the hypophyseal portal circulation, where it stimulates adrenocorticotropic hormone (ACTH) release from the anterior pituitary.

Functionally, CRH antibody identifies the peptide precursor prepro-CRH, which undergoes proteolytic cleavage to produce the mature active hormone. CRH binds to G protein-coupled receptors CRHR1 and CRHR2 on pituitary corticotrophs, activating adenylate cyclase and cAMP-dependent signaling pathways that induce ACTH synthesis and secretion. ACTH in turn stimulates cortisol release from the adrenal cortex, completing the neuroendocrine feedback loop that maintains stress adaptation and homeostasis.

The CRH gene is located on chromosome 8q13.1 and is primarily expressed in the paraventricular nucleus of the hypothalamus, but also in peripheral tissues such as the placenta, adrenal glands, and gastrointestinal tract. CRH coordinates endocrine, autonomic, and behavioral responses to stress, linking neuroendocrine signaling with emotional and metabolic regulation.

Pathologically, dysregulated CRH expression is associated with anxiety, depression, Cushingi 1/2s syndrome, and inflammatory disorders. Overproduction of CRH leads to chronic activation of the HPA axis and elevated cortisol levels, while deficiency impairs stress response and adrenal function. Research using CRH antibody supports studies in neuroendocrinology, stress physiology, and behavioral neuroscience.

CRH antibody is validated for immunohistochemistry, ELISA, and immunofluorescence to detect neuropeptides and hormones. NSJ Bioreagents provides CRH antibody reagents optimized for research in endocrine signaling, stress regulation, and hypothalamic function.

Structurally, Corticotropin releasing hormone is a 41-residue peptide containing a disulfide bond that stabilizes its helical structure, essential for receptor binding and biological activity. Its precursor includes a signal peptide directing secretion and prohormone regions involved in processing. This antibody enables investigation of CRH's role in HPA axis regulation, neuropeptide signaling, and stress-related disorders.

UniProt: P06850

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour