Recombinant Cyclin B1 anticorps (full length)

N° du produit ABIN7878062

Cet anticorps Lapin Monoclonal détecte spécifiquement Cyclin B1 dans ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Cyclin B1 anticorps (full length) (ABIN7878062)

Antigène: Cyclin B1 (CCNB1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CCNB1-7030R

Détail du produit anti-Cyclin B1 anticorps

Épitope: full length

Fonction: Cyclin B1 Antibody

Purification: Protein A/G affinity

Immunogène: Recombinant full-length human CCNB1 protein was used as the immunogen for the Cyclin B1 antibody.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal dilution of the Cyclin B1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the Cyclin B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: Cyclin B1

Sujet: It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase