MLH1 anticorps (full length)

N° du produit ABIN7878674

Cet anticorps Souris Monoclonal détecte spécifiquement MLH1 dans IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour MLH1 anticorps (full length) (ABIN7878674)

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLH1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MLH1-7560

Détail du produit anti-MLH1 anticorps

Épitope: full length

Fonction: MutL Homolog 1 Antibody / MLH1

Purification: Protein A/G affinity

Immunogène: Recombinant full-length human MLH1 protein was used as the immunogen for the MutL Homolog 1 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the MutL Homolog 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the MutL Homolog 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MutL Homolog 1

Sujet: This mAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.

UniProt: P40692

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response