Recombinant Phospholamban anticorps (pSer16)

N° du produit ABIN7879712

Cet anticorps Lapin Monoclonal détecte spécifiquement Phospholamban dans WB. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour Recombinant Phospholamban anticorps (pSer16) (ABIN7879712)

Antigène: Phospholamban (PLN)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Phospholamban est non-conjugé

Application: Western Blotting (WB)

Clone: 32P55

Détail du produit anti-Phospholamban anticorps

Épitope: pSer16

Fonction: Phospho-Phospholamban (S16) Antibody / PLN

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Phospho-Phospholamban (S16) was used as the immunogen for the Phospho-Phospholamban (S16) antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Phospho-Phospholamban (S16) antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Phospho-Phospholamban (S16) antibody at -20oC.

Détails sur Phospholamban

Antigène: Phospholamban (PLN)

Autre désignation: Phospholamban

Sujet: Phospho-Phospholamban (S16) antibody detects PHOSPHOLAMBAN, encoded by the PLN gene, when phosphorylated at serine 16. Alternate names include cardiac phospholamban, PLB, and phospholamban phosphoprotein. PHOSPHOLAMBAN is a small transmembrane protein in the sarcoplasmic reticulum of cardiac and skeletal muscle. It regulates SERCA2a, the sarcoplasmic reticulum Ca2+ ATPase responsible for calcium uptake during muscle relaxation. In its unphosphorylated state, PHOSPHOLAMBAN inhibits SERCA2a. Phosphorylation at serine 16 by PKA relieves this inhibition, enhancing calcium uptake, promoting relaxation, and increasing cardiac contractility.

Phospho-Phospholamban (S16) antibody is widely applied in cardiovascular biology, muscle physiology, and signaling research. By detecting phosphorylation at serine 16, researchers can study how beta adrenergic signaling enhances cardiac output through PKA mediated regulation of calcium cycling. This site specific antibody allows distinction between active and inhibited PHOSPHOLAMBAN, providing insights into heart function under physiological and pathological conditions.

Applications include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated PHOSPHOLAMBAN in heart lysates, immunohistochemistry maps phosphorylation in cardiac tissue sections, and immunofluorescence highlights localization in sarcoplasmic reticulum. ELISA assays allow quantification of phosphorylation status in experimental models. These techniques provide direct evidence of cardiac signaling events.

Dysregulation of PHOSPHOLAMBAN phosphorylation contributes to heart failure, arrhythmias, and cardiomyopathies. Mutations in PLN cause inherited dilated cardiomyopathy and arrhythmogenic right ventricular cardiomyopathy. Monitoring serine 16 phosphorylation provides biomarkers for disease progression and therapy efficacy. By applying Phospho-Phospholamban (S16) antibody, scientists can investigate how altered signaling leads to impaired cardiac relaxation and contractility.

Phosphorylation of PHOSPHOLAMBAN is also studied in exercise physiology, where beta adrenergic stimulation enhances contractility. The antibody supports research into how training, drugs, and genetic variants influence calcium cycling. Translational applications include evaluation of therapeutic interventions targeting SERCA2a regulation, such as gene therapy or pharmacologic modulators. NSJ Bioreagents offers Phospho-Phospholamban (S16) antibody with validated specificity, supporting robust research into cardiac physiology and disease.

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity