Recombinant PKC anticorps (pSer729)

N° du produit ABIN7879770

L’anticorps anti-PKC Monoclonal Lapin est utilisé pour la détection de PKC dans des échantillons de Humain et Rat. Il a été validé pour WB.

Aperçu rapide pour Recombinant PKC anticorps (pSer729) (ABIN7879770)

Antigène: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp PKC est non-conjugé

Application: Western Blotting (WB)

Clone: 32P75

Détail du produit anti-PKC anticorps

Épitope: pSer729

Fonction: Phospho-PKC (Ser729) Antibody / PRKCA/PRKCB/PRKCE

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Phospho-PKC (S729) was used as the immunogen for the Phospho-PKC (Ser729) antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Phospho-PKC (Ser729) antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Phospho-PKC (Ser729) antibody at -20oC.

Détails sur PKC

Antigène: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Autre désignation: PKC

Sujet: Phospho-PKC (Ser729) antibody detects protein kinase C when phosphorylated at serine 729. Protein kinase C belongs to a family of serine threonine kinases encoded by multiple genes including PRKCA, PRKCB, and PRKCG. Phosphorylation at Ser729 occurs within the hydrophobic motif of PKC alpha and related isoforms, a modification essential for stability and full catalytic activity. This phosphorylation is typically mediated by kinases such as PDK1 and mTORC2, which regulate PKC maturation and downstream signaling capacity.

Phospho-PKC (Ser729) antibody is widely applied in cancer biology, signal transduction, and neuroscience. PKC enzymes regulate cell proliferation, differentiation, apoptosis, and cytoskeletal remodeling. Phosphorylation at Ser729 serves as a marker for mature active PKC isoforms and is required for proper subcellular localization and substrate phosphorylation. By detecting phospho Ser729, researchers can measure activation status of PKC pathways and how they change in disease states.

Western blot assays reveal distinct phospho PKC bands corresponding to activated isoforms. Immunohistochemistry maps Ser729 phosphorylation in tissues such as brain, heart, and tumors. Immunofluorescence highlights dynamic translocation of PKC from cytosol to plasma membrane or other compartments upon stimulation. These methods allow precise characterization of PKC activation at the molecular and cellular level.

PKC activity is critical for oncogenic signaling. Dysregulation of phosphorylation at Ser729 is associated with altered PKC stability and oncogenic transformation. Certain cancers show reduced PKC activity due to mutations preventing proper phosphorylation, while others display hyperactivation that promotes tumor growth. By applying Phospho-PKC (Ser729) antibody, scientists can study how phosphorylation events regulate tumor biology and therapeutic response.

PKC enzymes are also central to neuronal signaling and plasticity. Phosphorylation at Ser729 supports synaptic transmission, long term potentiation, and memory formation. Altered PKC activity has been implicated in neurodegenerative diseases such as Alzheimer disease and Parkinson disease. With phospho specific antibody tools, researchers can dissect how PKC phosphorylation influences brain function and pathology.

In cardiology, PKC phosphorylation regulates cardiac hypertrophy, contractility, and response to ischemia. The Ser729 site provides a biomarker for assessing PKC function in cardiac stress and adaptation. This makes Phospho-PKC (Ser729) antibody relevant not only for molecular signaling studies but also for translational research into heart disease.

Phospho-PKC (Ser729) antibody from NSJ Bioreagents provides strong specificity for detecting active phosphorylated PKC isoforms. Its reliable performance across multiple applications ensures accurate monitoring of kinase activity in health and disease.

UniProt: Q02156

Pathways: Positive Regulation of Response to DNA Damage Stimulus