Recombinant DPYSL2 anticorps (pThr514)

N° du produit ABIN7879800

L’anticorps anti-DPYSL2 Monoclonal Lapin est utilisé pour la détection de DPYSL2 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB.

Aperçu rapide pour Recombinant DPYSL2 anticorps (pThr514) (ABIN7879800)

Antigène: DPYSL2 (Dihydropyrimidinase-Like 2 (DPYSL2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp DPYSL2 est non-conjugé

Application: Western Blotting (WB)

Clone: 32D10

Détail du produit anti-DPYSL2 anticorps

Épitope: pThr514

Fonction: Phospho-CRMP2 (Thr514) Antibody / Collapsin response mediator protein 2

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human Phospho-CRMP2 (T514) was used as the immunogen for the Phospho-CRMP2 (Thr514) antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Phospho-CRMP2 (Thr514) antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the Phospho-CRMP2 (Thr514) antibody at -20oC.

Détails sur DPYSL2

Antigène: DPYSL2 (Dihydropyrimidinase-Like 2 (DPYSL2))

Autre désignation: CRMP2

Sujet: Phospho-CRMP2 (Thr514) antibody detects collapsin response mediator protein 2 when phosphorylated at threonine 514. CRMP2, encoded by the DPYSL2 gene, is a cytosolic phosphoprotein that regulates axon guidance, neuronal polarity, and microtubule dynamics. Phosphorylation at Thr514, primarily mediated by GSK3 beta, reduces CRMP2 binding to tubulin and destabilizes microtubules. This modification plays critical roles in neuronal development and synaptic remodeling.

Phospho-CRMP2 (Thr514) antibody is widely applied in neuroscience research, particularly in studies of axon guidance and neurodegeneration. By detecting phosphorylated CRMP2, researchers can distinguish between active and inhibited states of the protein. Elevated phosphorylation of CRMP2 at Thr514 has been associated with Alzheimer disease and other neurodegenerative conditions where microtubule stability is compromised.

The antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, Phospho-CRMP2 (Thr514) antibody detects distinct phosphorylated isoforms. Immunohistochemistry highlights tissue specific phosphorylation patterns in brain regions, while immunofluorescence enables visualization of phospho CRMP2 at growth cones and synapses. These applications make the antibody valuable for developmental neuroscience and disease studies.

Phosphorylation of CRMP2 at Thr514 regulates not only axonal outgrowth but also dendritic spine morphology and synaptic plasticity. Dysregulation of this site contributes to cognitive decline and neuropsychiatric disorders. By applying Phospho-CRMP2 (Thr514) antibody, scientists can explore mechanisms linking abnormal phosphorylation to neuronal dysfunction and identify therapeutic approaches that target CRMP2 signaling.

Phospho-CRMP2 (Thr514) antibody from NSJ Bioreagents provides reliable specificity for detecting phosphorylated DPYSL2, enabling accurate monitoring of microtubule regulatory pathways in health and disease.

UniProt: Q16555

Pathways: Regulation of Cell Size