ATG4A anticorps

N° du produit ABIN7880031

L’anticorps anti-ATG4A Monoclonal Souris est utilisé pour la détection de ATG4A dans des échantillons de Humain. Il a été validé pour WB, IF, FACS et IHC (p).

Aperçu rapide pour ATG4A anticorps (ABIN7880031)

Antigène: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATG4A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1458CT808-66-25-69

Détail du produit anti-ATG4A anticorps

Fonction: ATG4A Antibody

Purification: Protein G affinity

Immunogène: Recombinant human protein was used as the immunogen for the ATG4A antibody.

Isotype: IgG2b, kappa

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the ATG4A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot the ATG4A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur ATG4A

Antigène: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Autre désignation: ATG4A

Sujet: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.

UniProt: Q8WYN0

Pathways: Autophagy