BrdU anticorps

N° du produit ABIN7880101

Cet anticorps anti-BrdU Monoclonal Souris (Clone SPM537) (ABIN7880101) détecte spécifiquement BrdU dans IHC (p).

Aperçu rapide pour BrdU anticorps (ABIN7880101)

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BrdU est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM537

Détail du produit anti-BrdU anticorps

Fonction: Anti-BrdU Antibody

Purification: Protein G affinity chromatography

Immunogène: Bromodeoxyuridine was used as the immunogen for this anti-BrdU antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: The optimal dilution of the anti-BrdU antibody for each application should be determined by the researcher.

1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with Trypsin at 1 mg/mL PBS, 10 min at 37oC.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the anti-BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur BrdU

Antigène: BrdU (Bromodeoxyuridine (BrdU))

Autre désignation: BrdU

Classe de substances: Chemical

Sujet: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.