Caldesmon anticorps

N° du produit ABIN7880139

L’anticorps anti-Caldesmon Monoclonal Souris est utilisé pour la détection de Caldesmon dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour Caldesmon anticorps (ABIN7880139)

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caldesmon est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM168

Détail du produit anti-Caldesmon anticorps

Fonction: Caldesmon Antibody / CALD1 / CAD (HMW)

Purification: Protein G affinity chromatography

Immunogène: Crude human uterus extract was used as the immunogen for the Caldesmon antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the Caldesmon antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Caldesmon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Caldesmon

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Autre désignation: Caldesmon

Sujet: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction