CA9 anticorps

N° du produit ABIN7880169

L’anticorps anti-CA9 Monoclonal Souris est utilisé pour la détection de CA9 dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour CA9 anticorps (ABIN7880169)

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CA9 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM487

Détail du produit anti-CA9 anticorps

Fonction: Carbonic Anhydrase IX Antibody

Purification: Protein G affinity chromatography

Immunogène: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX antibody.

Isotype: IgG2b, kappa

Information d'application

Indications d'application: The optimal dilution of the Carbonic Anhydrase IX antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Carbonic Anhydrase IX antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CA9

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Autre désignation: Carbonic Anhydrase IX

Sujet: Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/CA9/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

UniProt: Q16790