CA9 anticorps

N° du produit ABIN7880170

Cet anticorps anti-CA9 Monoclonal Souris (Clone SPM314) (ABIN7880170) détecte spécifiquement CA9 dans IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CA9 anticorps (ABIN7880170)

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CA9 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM314

Détail du produit anti-CA9 anticorps

Fonction: CA9 Antibody

Purification: Protein G affinity chromatography

Immunogène: The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this CA9 antibody.

Isotype: IgG2b, kappa

Information d'application

Indications d'application: The optimal dilution of the CA9 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CA9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CA9

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Autre désignation: CA9

Sujet: Recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/CA9/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CA9 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CA9 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This mAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

UniProt: Q16790