Recombinant AER61 anticorps

N° du produit ABIN7880438

Cet anticorps Lapin Monoclonal détecte spécifiquement AER61 dans WB. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Recombinant AER61 anticorps (ABIN7880438)

Antigène: AER61 (C3orf64) (Chromosome 3 Open Reading Frame 64 (C3orf64))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp AER61 est non-conjugé

Application: Western Blotting (WB)

Clone: 30E02

Détail du produit anti-AER61 anticorps

Fonction: AER61 Antibody / EOGT

Purification: Affinity-chromatography

Immunogène: A synthesized peptide derived from human AER61 was used as the immunogen for the EOGT antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the EOGT antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the EOGT antibody at -20oC.

Détails sur AER61

Antigène: AER61 (C3orf64) (Chromosome 3 Open Reading Frame 64 (C3orf64))

Autre désignation: AER61

Sujet: EOGT antibody detects EGF domain specific O linked N acetylglucosamine transferase, an enzyme encoded by the EOGT gene. EOGT catalyzes the addition of O GlcNAc moieties to EGF like domains of secreted and membrane proteins, modifying signaling molecules and receptors. This post translational modification regulates ligand receptor interactions and signaling pathways critical for development and homeostasis.

EOGT antibody is widely used in studies of glycosylation, signaling, and developmental biology. By detecting EOGT, researchers can explore how O GlcNAc modification influences Notch signaling, growth factor binding, and tissue morphogenesis. Mutations in EOGT are associated with Adams Oliver syndrome, a developmental disorder characterized by limb and scalp defects, underscoring its biological importance.

In western blot assays, EOGT antibody detects protein bands corresponding to the enzyme in tissue and cell extracts. Immunohistochemistry maps expression in developing tissues, while immunofluorescence reveals subcellular localization consistent with endoplasmic reticulum and Golgi compartments. These methods support detailed analysis of glycosyltransferase biology.

EOGT mediated O GlcNAc modification contributes to signaling regulation in vascular and neural development. Dysregulation of this pathway has implications in congenital disorders, cancer, and immune regulation. By applying EOGT antibody, scientists can investigate how defects in protein glycosylation contribute to human disease and identify therapeutic strategies that target glycosylation pathways.

EOGT antibody from NSJ Bioreagents provides strong specificity for studying glycosylation and developmental biology. Its performance across multiple assays makes it a valuable reagent for exploring the role of O GlcNAc transferases in health and disease.

UniProt: Q5NDL2