Crossover junction endonuclease EME1 (EME1) anticorps

N° du produit ABIN7880514

L’anticorps anti-Crossover junction endonuclease EME1 Monoclonal Souris est utilisé pour la détection de Crossover junction endonuclease EME1 dans des échantillons de Humain. Il a été validé pour WB et IHC (p).

Aperçu rapide pour Crossover junction endonuclease EME1 (EME1) anticorps (ABIN7880514)

Antigène: Crossover junction endonuclease EME1 (EME1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MTA31 7H2-1

Détail du produit

Fonction: EME1 Antibody

Purification: Protein G affinity chromatography

Immunogène: His-tagged recombinant Eme1 of human origin was used as the immunogen for the EME1 antibody.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the EME1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the EME1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détail du antigène

Antigène: Crossover junction endonuclease EME1 (EME1)

Autre désignation: EME1

Sujet: Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3 DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.

UniProt: Q96AY2