MGMT anticorps

N° du produit ABIN7881870

Cet anticorps Souris Monoclonal détecte spécifiquement MGMT dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour MGMT anticorps (ABIN7881870)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MGMT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: MGMT-7454

Détail du produit anti-MGMT anticorps

Fonction: MGMT Antibody (azide and preservative free)

Purification: Protein A/G affinity

Immunogène: Purified His-tagged MGMT protein was used as the immunogen for the MGMT antibody.

Isotype: IgG2, kappa

Information d'application

Indications d'application: Optimal dilution of the MGMT antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: -20 °C

Stockage commentaire: Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur MGMT

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

Sujet: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O6- alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6- position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant.

UniProt: P16455

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus