ADAM10 anticorps (Extracellular, N-Term)

N° du produit ABIN7884716

Cet anticorps anti-ADAM10 Polyclonal Lapin (ABIN7884716) détecte spécifiquement ADAM10 dans WB, IHC, FACS et LCI. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ADAM10 anticorps (Extracellular, N-Term) (ABIN7884716)

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADAM10 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Classe de qualité: Carrier-free

Détail du produit anti-ADAM10 anticorps

Épitope: AA 400-414, Extracellular, N-Term

Fonction: A Rabbit Polyclonal Antibody to ADAM10 (extracellular)

Homologie: Rat,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogène: CTPGESKNLGQKENG, corresponding to amino acid residues 400 - 414 of mouse ADAM10

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200-1:500

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7234615)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur ADAM10

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Autre désignation: Disintegrin And Metalloproteinase Domain-Containing Protein 10

Sujet:

Synonyms: Disintegrin And Metalloproteinase Domain-Containing Protein 10, ADAM Metallopeptidase Domain 10, CD156c

Description: Disintegrin and metalloproteinase domain-containing protein 10, also known as ADAM10, is a membrane-anchored protease that belongs to the ADAM protease family. ADAMs are type I transmembrane proteins with a large extracellular domain and a shorter cytosolic part, they contain a metalloprotease domain, a disintegrin domain, a cystein-rich domain, an EGF-like domain, a transmembrane domain and a cytoplasmic tail2. 37 murine and 22 human ADAMs are known and only some of them contain the catalytically relevant domain (HEXGHXXGXXHD) and are therefore able to cleave the extracellular domains of several membrane-bound proteins in a process known as 'ectodomain shedding'1. This process represents a unique and irreversible posttranslational event regulating the function and half-life of many intracellular and extracellular proteins and hence the ADAM family protein's activity is essential for regulation of various cellular functions2. ADAM10 has many substrates, among which are cellular adhesion molecules such as cadherin, receptors and ligands3. it is also capable of anti-amyloidogenic proteolysis of the amyloid precursor protein, this is why ADAM10 mutations were shown to be correlated with Alzheimer's disease2. In addition, it was shown to involved in various human diseases and disorders such as Huntington's disease, psoriasis, lupus, and multiple types of cancer. The central role of ADAM10 in various cell processes and diseases reveals the potency of ADAM10-targeting drug development as a future therapeutic option2.

ID gène: 11487

UniProt: O35598

Pathways: Signalisation Notch, EGFR Signaling Pathway